Recombinant:Article Title: The Negative Cross-Talk between SAG/RBX2/ROC2 and APC/C E3 Ligases in Regulation of Cell Cycle Progression and Drug Resistance
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SAG home-made (Jia et al., 2010) N/A Rabbit Polyclonal anti-APC2 Cell Signaling Cat# 12301s; RRID:AB_2619505 Rabbit monoclonal anti-APC11 Cell Signaling Cat# 14090s; RRID:AB_485228 Rabbit monoclonal anti-CyclinB1 Cell Signaling Cat# 12231; RRID:AB_369073 Rabbit monoclonal anti-Securin Cell Signaling Cat# 13445; RRID:AB_564009 Rabbit monoclonal anti-Aurora A Cell Signaling Cat# 91590s; RRID:AB_451072 Rabbit monoclonal anti-PLK1 Cell Signaling Cat# 4513; RRID:AB_613880 Rabbit monoclonal anti-PhosphorylationH3(ser10) Cell Signaling Cat# 3377p; RRID:AB_10688281 Mouse monoclonal anti-Cyclin A Cell Signaling Cat# 4656P; RRID:AB_563675 Mouse monoclonal anti-UBE2C Santa Cruz Cat# sc271050; RRID:AB_11215373 Mouse monoclonal anti-CDC20 Santa Cruz Cat# sc13162; RRID:AB_2244441 Rabbit polyclonal anti-UBE2S Abcam Cat# ab177508; RRID:AB_2211467 Rabbit polyclonal anti-CDH1 Abcam Cat# ab217038; RRID:AB_10650758 Mouse monoclonal anti-FLAG Sigma Cat# F1804-200UG; RRID:AB_1537400 Mouse monoclonal anti-p27 BD Biosciences Cat# BD554069; RRID:AB_563927 Chemicals, Peptides, and Recombinant Proteins MG132 Sigma Cat#: M7449 Palbociclib Selleckchem Cat#: S1116 Thymidine Sigma Cat#: T1895 Nocodazole Sigma Cat#: M1404 Taxol Selleckchem Cat#: S115010 RO3306 R&D systems Cat#: 4181 Paclitaxel FRESENIUS KABI, Cat#: 760305 Pro-TAME R&D systems Cat#: I-440-01M Critical Commercial Assays ATPlite Luminescence Assay Kit PerkinElmer Cat#: 6016943 Experimental Models: Cell Lines H1299 American Type Culture Collection (ATCC) Cat# NCI-H1299 ATCC CRL-5803 .. HEK293 ATCC Cat# 293 [HEK293] ATCC CRL-1573 HeLa ATCC Cat# HeLa ATCC CCL-2 A549 ATCC Cat# A549 ATCC CCL-185 A427 ATCC Cat# A-427 ATCC HTB-53 H1299: lentivirus-based siRNA knockdown of SAG (Lt-SAG), along with scrambled siRNA control (Lt-Cont) Tan et al., 2016 N/A Oligonucleotides siRNA targeting sequence: APC2: TGCGCGGAGTCTTGTTCTTTA Genepharma N/A siRNA targeting sequence: APC11: GACCATTCATGTGACCTTTTTGG Genepharma N/A (Continued on next page) e1 Cell Reports 32, 108102, September 8, 2020
Luminescence Assay:Article Title: The Negative Cross-Talk between SAG/RBX2/ROC2 and APC/C E3 Ligases in Regulation of Cell Cycle Progression and Drug Resistance
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SAG home-made (Jia et al., 2010) N/A Rabbit Polyclonal anti-APC2 Cell Signaling Cat# 12301s; RRID:AB_2619505 Rabbit monoclonal anti-APC11 Cell Signaling Cat# 14090s; RRID:AB_485228 Rabbit monoclonal anti-CyclinB1 Cell Signaling Cat# 12231; RRID:AB_369073 Rabbit monoclonal anti-Securin Cell Signaling Cat# 13445; RRID:AB_564009 Rabbit monoclonal anti-Aurora A Cell Signaling Cat# 91590s; RRID:AB_451072 Rabbit monoclonal anti-PLK1 Cell Signaling Cat# 4513; RRID:AB_613880 Rabbit monoclonal anti-PhosphorylationH3(ser10) Cell Signaling Cat# 3377p; RRID:AB_10688281 Mouse monoclonal anti-Cyclin A Cell Signaling Cat# 4656P; RRID:AB_563675 Mouse monoclonal anti-UBE2C Santa Cruz Cat# sc271050; RRID:AB_11215373 Mouse monoclonal anti-CDC20 Santa Cruz Cat# sc13162; RRID:AB_2244441 Rabbit polyclonal anti-UBE2S Abcam Cat# ab177508; RRID:AB_2211467 Rabbit polyclonal anti-CDH1 Abcam Cat# ab217038; RRID:AB_10650758 Mouse monoclonal anti-FLAG Sigma Cat# F1804-200UG; RRID:AB_1537400 Mouse monoclonal anti-p27 BD Biosciences Cat# BD554069; RRID:AB_563927 Chemicals, Peptides, and Recombinant Proteins MG132 Sigma Cat#: M7449 Palbociclib Selleckchem Cat#: S1116 Thymidine Sigma Cat#: T1895 Nocodazole Sigma Cat#: M1404 Taxol Selleckchem Cat#: S115010 RO3306 R&D systems Cat#: 4181 Paclitaxel FRESENIUS KABI, Cat#: 760305 Pro-TAME R&D systems Cat#: I-440-01M Critical Commercial Assays ATPlite Luminescence Assay Kit PerkinElmer Cat#: 6016943 Experimental Models: Cell Lines H1299 American Type Culture Collection (ATCC) Cat# NCI-H1299 ATCC CRL-5803 .. HEK293 ATCC Cat# 293 [HEK293] ATCC CRL-1573 HeLa ATCC Cat# HeLa ATCC CCL-2 A549 ATCC Cat# A549 ATCC CCL-185 A427 ATCC Cat# A-427 ATCC HTB-53 H1299: lentivirus-based siRNA knockdown of SAG (Lt-SAG), along with scrambled siRNA control (Lt-Cont) Tan et al., 2016 N/A Oligonucleotides siRNA targeting sequence: APC2: TGCGCGGAGTCTTGTTCTTTA Genepharma N/A siRNA targeting sequence: APC11: GACCATTCATGTGACCTTTTTGG Genepharma N/A (Continued on next page) e1 Cell Reports 32, 108102, September 8, 2020
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